Evaluation of cotton rats as a model for severe acute respiratory syndrome.
Identifieur interne : 003114 ( Main/Exploration ); précédent : 003113; suivant : 003115Evaluation of cotton rats as a model for severe acute respiratory syndrome.
Auteurs : D M Watts [États-Unis] ; C J Peters ; P. Newman ; N. Wang ; N. Yoshikawa ; C K Tseng ; P R WydeSource :
- Vector borne and zoonotic diseases (Larchmont, N.Y.) [ 1530-3667 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Syndrome respiratoire aigu sévère.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Femelle, Modèles animaux de maladie humaine, Mâle, Projets pilotes, Réplication virale, Sigmodontinae.
English descriptors
- KwdEn :
- MESH :
- pathology : Severe Acute Respiratory Syndrome.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Female, Male, Pilot Projects, Sigmodontinae, Vero Cells, Virus Replication.
Abstract
Experimental studies were conducted to evaluate two species of cotton rats, Sigmodon hispidus and Sigmodon fulviventer, as a model for severe acute respiratory syndrome (SARS). Blood and turbinate wash samples, and lung tissue were collected from each animal at different time points after SARS coronavirus (CoV) infection for determining the growth curve of virus, if any, by the standard infectivity assay in Vero E6 cells. In addition, sections of the lung, liver, spleen, and kidney were taken and used for histology analysis. All animals were observed daily for signs of illness, and in some experiments, animals were weighed on the day when they were sacrificed. The results indicated that the cotton rat species, S. hispidus and S. fulviventer, were not a useful model for either SARS-CoV infection or disease. This observation was supported by the absence of any signs of illness, the failure to consistently demonstrate virus in the blood and tissues, and the absent of any notable histopathology. However, infected animals were capable of producing neutralizing antibodies against SARS-CoV, suggesting the seroconversion did occur. Further studies are warranted to consider other animal species in efforts to find better animal models for the evaluation of SARS-CoV vaccines and antiviral drugs.
DOI: 10.1089/vbz.2007.0210
PubMed: 18447621
Affiliations:
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Newman</name></author><author><name sortKey="Wang, N" sort="Wang, N" uniqKey="Wang N" first="N" last="Wang">N. Wang</name></author><author><name sortKey="Yoshikawa, N" sort="Yoshikawa, N" uniqKey="Yoshikawa N" first="N" last="Yoshikawa">N. Yoshikawa</name></author><author><name sortKey="Tseng, C K" sort="Tseng, C K" uniqKey="Tseng C" first="C K" last="Tseng">C K Tseng</name></author><author><name sortKey="Wyde, P R" sort="Wyde, P R" uniqKey="Wyde P" first="P R" last="Wyde">P R Wyde</name></author></analytic><series><title level="j">Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><idno type="ISSN">1530-3667</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Disease Models, Animal</term><term>Female</term><term>Male</term><term>Pilot Projects</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Sigmodontinae</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Projets pilotes</term><term>Réplication virale</term><term>Sigmodontinae</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Disease Models, Animal</term><term>Female</term><term>Male</term><term>Pilot Projects</term><term>Sigmodontinae</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Projets pilotes</term><term>Réplication virale</term><term>Sigmodontinae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Experimental studies were conducted to evaluate two species of cotton rats, Sigmodon hispidus and Sigmodon fulviventer, as a model for severe acute respiratory syndrome (SARS). Blood and turbinate wash samples, and lung tissue were collected from each animal at different time points after SARS coronavirus (CoV) infection for determining the growth curve of virus, if any, by the standard infectivity assay in Vero E6 cells. In addition, sections of the lung, liver, spleen, and kidney were taken and used for histology analysis. All animals were observed daily for signs of illness, and in some experiments, animals were weighed on the day when they were sacrificed. The results indicated that the cotton rat species, S. hispidus and S. fulviventer, were not a useful model for either SARS-CoV infection or disease. This observation was supported by the absence of any signs of illness, the failure to consistently demonstrate virus in the blood and tissues, and the absent of any notable histopathology. However, infected animals were capable of producing neutralizing antibodies against SARS-CoV, suggesting the seroconversion did occur. Further studies are warranted to consider other animal species in efforts to find better animal models for the evaluation of SARS-CoV vaccines and antiviral drugs.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="Newman, P" sort="Newman, P" uniqKey="Newman P" first="P" last="Newman">P. Newman</name><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name><name sortKey="Tseng, C K" sort="Tseng, C K" uniqKey="Tseng C" first="C K" last="Tseng">C K Tseng</name><name sortKey="Wang, N" sort="Wang, N" uniqKey="Wang N" first="N" last="Wang">N. Wang</name><name sortKey="Wyde, P R" sort="Wyde, P R" uniqKey="Wyde P" first="P R" last="Wyde">P R Wyde</name><name sortKey="Yoshikawa, N" sort="Yoshikawa, N" uniqKey="Yoshikawa N" first="N" last="Yoshikawa">N. Yoshikawa</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Watts, D M" sort="Watts, D M" uniqKey="Watts D" first="D M" last="Watts">D M Watts</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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